Preservación hipotérmica del intestino delgadoun estudio morfológico mediante microscopía electrónica de barrido

Abstract

Introduction: The aim of this study is to analyze the effect of simple crystalloid solutions (saline and Ringer lactate solution) and an organ preservation solution (Celsior) on murine intestinal ileal mucosa by scanning electron microscopy after hypothermic preservation. Material and Methods : The intestinal segments of ileum from CD1 mice were intravascularly and intraluminally flushed with 3 different preservation solutions. The used solutions were the simple crystalloid solution normal saline -group 2- and Lactated Ringer (LR) -group 3-. As organ preservation solution, the solution Celsior -group 4- was used. The segments were stored for 14 hours at 4 ° C with the same solution used for perfusion. The samples were processed using standard protocols for scanning electron microscopy. Results: Our results show that group 2 was characterized by villous and microvilli pattern similar to the control group (group 1). The group 3 showed focal lesions characterized by missing epithelium at the apex level and the exposure of the connective-vascular axis of the villi. Finally, group 4 presented a disrupted villous pattern with extensive subepithelial clefts as well as complete denuded villi. Discussion: This study shows that simple crystalloid solutions administered via vascular and intraluminal maintain morphological integrity of the intestinal mucosa after hypothermic preservation. In addition, scanning electron microscopy is an optimal methodology to evaluate the topographical tissue damage induced by various preservation protocols.