Compuestos perfluorados en equinodermos marinos: Metodología analítica para su determinación y monitorización
- Julia Martín
- Juan Luis Santos
- Irene Aparicio
- Esteban Alonso
- Alberto Zafra-Gómez
- José Luis Vílchez
- Ana M. Beltrán (ed. lit.)
- Manuel Félix (ed. lit.)
Publisher: 3ciencias
ISBN: 978-84-948577-6-8
Year of publication: 2018
Pages: 159-167
Type: Book chapter
Abstract
In recent years endocrine disruptors have come into the spotlight of environmental and ecotoxicological studies. Among them, perfluorinated compounds stand out, both, for their wide industrial and domestic use and for their elevated estrogenic activity, showing adverse effects at trace level. They are released into the environment through industrial waste and wastewater discharges affecting to marine organisms, where they can accumulate. To contribute to this goal, this work proposes an analytical method for the simultaneous determination of six perfluorinated compounds in marine organisms (Holothuria tubulosa) and an environmental monitoring program is carried out in these organisms. The sample treatment involve steps of solvent extraction and clean-up of the extracts with dispersive sorbents prior to liquid chromatography–tandem mass spectrometry analysis. Recoveries between 84 and 101 %, precision (RSD < 9 %) and limits of quantification below 0.03 ng/g dry weight (d.w.) were achieved. All tested samples were positive in the analysis of contaminants with concentrations between 667 ng/g (dw) for perflorooctanoic acid (PFOA) and 0.81 ng/g (dw) for perfloropentanoic acid (PFPeA). In general, perfluorinated compounds of larger fluorocarbonated chain were quantified at higher concentration levels than those of shorter one.