Generación y caracterización de un modelo lesional corneal ex vivo para su uso en ingeniería tisular

  1. González - Andrades, E. 1
  2. Ionescu, A M 1
  3. Cardona, J C 1
  4. Ghinea, R 1
  5. Garzón, I 1
  6. Carriel, V. 1
  7. González - Andrades, M. 1
  1. 1 Universidad de Granada
    info

    Universidad de Granada

    Granada, España

    ROR https://ror.org/04njjy449

Revista:
Actualidad médica

ISSN: 0365-7965

Año de publicación: 2016

Tomo: 101

Número: 797

Páginas: 7-12

Tipo: Artículo

DOI: 10.15568/AM.2016.797.OR01 DIALNET GOOGLE SCHOLAR lock_openDIGIBUG editor

Otras publicaciones en: Actualidad médica

Objetivos de desarrollo sostenible

Resumen

Purpose: This study aims to generate and characterize an ex vivo corneal injury model, in order to assess the feasibility of artificial tissues created by using tissue engineering through the application of a corneal alkali burn with NaOH on porcine corneas. Methods: We conducted a descriptive study taking 18 eyes of adult pigs immediately after their death. Eyes were introduced on 1.5M NaOH for 1, 2, 3 and 5 minutes. After, they were washed with running water (5 min) and rapid washing with PBS, followed by a 2 minute wash in PBS was performed. Two control groups were included: untreated corneas and another corneas were performed running water washes and PBS whashes. Samples were processed for histological analysis by light microscopy with hematoxylin and eosin, alcian blue and picrosirius staining. The significance of the results was determined by ANOVA statistical analysis. Results: We observed a loss of endothelium and the corneal epithelium in all groups treated with NaOH. A tendency to compaction of collagen fibers after treatment with NaOH and picrosirius stain loss of green coloration fibers in samples treated with NaOH with respect to controls was also observed. Despite lower intensity observed in alcian blue staining in the posterior region compared to the other two and between samples treated with NaOH, no significant differences were found in this area. Conclusions: it has created a single lesion model, low cost and does not involve ethical issues, to assess the tissue created by Tissue Engineering.

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