Hyaluronidase pre-treatment enhances collagen staining in sturgeon notochord

  1. García-García,O.
  2. Chato-Astrain,J.
  3. Irastorza-Lorenzo,A.
  4. Durand-Herrera,D.
  5. Campos,F.
  6. Sánchez-Porras,D.
  7. Domezáin,A.
  8. Carmona,R.
  9. Alaminos,M.
  10. Carriel,V.
Revue:
Actualidad médica

ISSN: 0365-7965

Année de publication: 2018

Tomo: 103

Número: 804

Pages: 72-75

Type: Article

DOI: 10.15568/AM.2018.804.OR03 DIALNET GOOGLE SCHOLAR lock_openAccès ouvert editor

D'autres publications dans: Actualidad médica

Résumé

Objective: The current study aimed to design a histological method to determine the presence and organization of the collagen network in sturgeon notochord. Methods: Serial sections of sturgeon notochord (Acipenser naccarii) were used and assigned to two different experimental groups: Hyaluronidase pre-treatment (HP) in an alcohol acid solution (1% HCl in 70% alcohol solution) for 15 min and hyaluronidase solution in a 2 µg/ ml concentration (pre-heated at 37º C), and control (CTR) group, without pre-treatment. Then, the ECM was assessed by two histochemical methods: Picrosirius Red (PR) staining for 30 min with Sirius red (0.1% of Sirius red in saturated aqueous picric acid), for collagen bundle staining, and Alcian Blue (AB) staining for glycosaminoglycans detection. Results: Samples analyzed in this study showed positive histochemical reaction for collagen fibers in both experimental groups. Referring to PR staining, the CTR group presented a larger and homogeneous reaction was observed in the entire samples, whereas HP group presented a more definite and intense pattern of collagen network. Also, this more intense signal in HP group matched with an increase of birefringence in polarized microscopy images of PR. However, HP group showed a lower intense and more heterogeneous signal when was compared with CTR group in AB staining. Conclusion: Using a simple histological example, our study illustrates the capability of a hyaluronidase pre-treatment to enhance picrosirius red staining in sturgeon notochord trough light and polarized microscopy.

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