Regulación postraduccional de la respuesta de las plantas a volátiles emitidos por hongos fitopatógenos

Resumen

In this work I have investigated the role played by two post-translational regulatory mechanisms (e.g. redox regulation and proteostatic regulation) in the response of plants to the VCs emitted by the phytopathogenic fungus Alternaria alternata. In Chapter I of this work I hypothesized that plants´ responses to microbial VCs involve post-translational modifications of the thiol redox proteome through action of the plastidial NADPH-dependent thioredoxin reductase C (NTRC), which regulates chloroplast redox status via its functional relationship with 2-Cys peroxiredoxins. To test this hypothesis, I analyzed developmental, metabolic, hormonal, genetic and redox proteomic responses of wild type (WT) plants and a NTRC knock out mutant (ntrc) to VCs emitted by A. alternata. In Chapter II, I hypothesized that plants’ responses to microbial VCs involve post-translational modulation of the expression of enzymes of the 2-C-methyl-D-erythritol 4-phosphate (MEP) and shikimate pathways via mechanisms involving redox-activated photosynthesis signaling. To test this hypothesis, I compared the growth, metabolic, and proteomic responses in wild-type (WT) Arabidopsis plants and a cfbp1 mutant defective in a redox-regulated isoform of the Calvin-Benson cycle enzyme fructose-1,6-bisphosphatase to VCs emitted by A. alternata.