Evaluación de la proliferación de células adiposas en andamios de fibrina y quitosan: Revisión sistemática

  1. Alfonso Rodríguez, Camilo Andrés
  2. González Colmenares, Gretel
  3. Dávila, Adriana
  4. Marín, Claudia
  5. Villamarín, Sandra
  6. Garzón, Hernán Santiago
  7. Alaminos, Miguel
Revista:
Actualidad médica

ISSN: 0365-7965

Año de publicación: 2020

Tomo: 105

Número: 811

Páginas: 209-220

Tipo: Artículo

DOI: 10.15568/AM.2020.811.REV02 DIALNET GOOGLE SCHOLAR lock_openAcceso abierto editor

Otras publicaciones en: Actualidad médica

Resumen

Introducción: Actualmente las matrices son utilizadas para la regeneración de órganos y tejidos. Dentro de los polímeros naturales más usados están el quitosán y la fibrina. Estas matrices proporcionan un am- biente tridimensional, con características biome-cánicas adecuadas, las cuales permiten la proliferación, migración y diferenciación ce-lular. El objetivo del presente estudio fue realizar una revisión sistemática de aquellos trabajos científicos que tratan de “evaluar la capacidad de proliferación in vitro de célu-las madre adiposas sobre andamios de fibrina y quitosán”. Materiales y Métodos: Se realizó una búsqueda bibliográfica en las bases de datos PubMed, Proquest y Science direct, utilizando términos de búsqueda específicos, desde enero de 2006 hasta di-ciembre de 2019. Dos investigadores determinaron independientemente la elegibilidad de los estudios. Se rea- lizó la preselección de los artículos teniendo en cuenta los cri-terios de inclusión y exclusión. De los artículos seleccionados se extrajo información sobre morfología celular, comportamiento del cambio dimensional del polímero, aisla-miento y caracterización celular, crecimiento, viabilidad, tasa de super- vivencia y eva-luación /citotóxica de las células; cuando faltaron datos, se contactó con los autores de la publicación. Se tuvo en cuenta la descripción de los métodos para obtener datos de proliferación celular mediante pruebas metabólicas como: (MTT), (MTS), WST-1, LIVE-DEAD, microscopía electróni- ca de barrido, microscopia electrónica de transmisión o microscopia de fluorescencia. Se utilizó una lista de chequeo para la valoración de los ítems y se determinó el índice de concordancia para los dos investigadores. Resulta-dos: La búsqueda inicial arrojó 165 publicaciones, de los cuales se excluyeron 100 por no cumplir con los criterios de inclusión. Sesenta y cinco se revisaron a texto completo, 58 fueron excluidos por encontrarse duplicados, presentar combinación de quitosán y fibrina con otros compuestos o por no encontrarse toda la información para la evalua-ción de la rigurosidad me - todológica. Finalmente, siete artículos fueron seleccionados y se sometieron a la evaluación metodológica. Conclusión: Los estudios seleccionados demuestran las ventajas biológicas y mecánicas de los biomateriales de fibrina y quito-sán y su capacidad para estimular la proliferación celular. Estos biomateriales podrían ser considerados como opciones para ser utilizados como matrices en pro- tocolos de ingeniería tisular y medicina regenerativa. Futuras investigaciones sobre evaluación de la proliferación celular sobre cualquier biomaterial deben estar direccionadas en plan-tear rigurosa metodología que permita la obtención de resultados fiables.

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Fuente de los datos: Dialnet