Aplicación de las técnicas de medida de fluorescencia para el estudio de la fotosíntesis en la enseñanza práctica de Ecofisiología Vegetal

  1. Asma Boujenna
  2. Vanessa Martos Núñez
  3. Belén García del Moral Garrido
  4. Luis F. García del Moral
Revista:
ReiDoCrea: Revista electrónica de investigación y docencia creativa

ISSN: 2254-5883

Any de publicació: 2022

Volum: 11

Pàgines: 167-170

Tipus: Article

DOI: 10.30827/DIGIBUG.73455 DIALNET GOOGLE SCHOLAR lock_openAccés obert editor

Altres publicacions en: ReiDoCrea: Revista electrónica de investigación y docencia creativa

Resum

The kinetics of fluorescence emission by chlorophyll is of great interest not only for research purposes, but also for the study of the photochemistry of photosynthesis and its alteration by stress factors, since it provides information about the state of photochemical reactions Under optimal conditions of the photosynthetic apparatus, the energy captured by chlorophylls is used, with great efficiency, in the primary photochemical reactions of photosynthesis (more than 90%) and very little is dissipated in the form of fluorescence. Therefore, an increase in fluorescence emission compared to the basal level always implies an alteration in the photosynthetic process. The objective of this work is to contribute to the practical knowledge by the students of a Plant Ecophysiology course of the applications of fluorescence emission by leaves to the knowledge of the photosynthetic process.

Referències bibliogràfiques

  • Bolhàr-Nordenkampf HR, & Öquist G (1993). Chlorophyll fluorescence as a tool in photosynthesis research. In: Hall DO., Scurlock JMO, Bolhàr-Nordenkampf H, Leegood RC, Long SP (eds). Photosynthesis and Production in a Changing Environment. Springer, Dordrecht. https://doi.org/10.1007/978-94-011-1566-7_12
  • Genty B, Briantais JM, & Baker, NR (1989). The relationship between the quantum yield of photosynthetic electron transport and quenching of chlorophyll fluorescence. Biochim Biophys Acta 990: 87-92. https://doi.org/10.1016/S0304-4165(89)80016-9
  • Krall JP, & Edwards GE. (2006). Relationship between PSI and PSII activity and CO2 fixation in leaves. Physiol Plant 86: 180-187. https://doi.org/10.1111/j.1399-3054.1992.tb01328.x
  • Krause GH, & Weis E (1991). Chlorophyll Fluorescence and Photosynthesis: Annual Review of Plant Physiology and Plant Molecular Biology, 42(1), 313-349. https://doi.org/10.1146/annurev.pp.42.060191.001525
  • Lambers H, Stuart Chapin, F & Pons TL (2008). Plant Physiological Ecology. Second edition. Springer, New York. https://doi.org/10.2307/176572
  • Murchie EH, & Lawson T (2013). Chlorophyll fluorescence analysis: a guide to good practice and understanding some new applications Journal of Experimental Botany, 64(13), 3983–3998. https://doi.org/10.1093/jxb/ert208
  • Schreiber U, Bilger W, & Neubauer C (1995) Chlorophyll Fluorescence as a Nonintrusive Indicator for Rapid Assessment of In Vivo Photosynthesis. In: Schulze ED, & Caldwell MM (eds). Ecophysiology of Photosynthesis. Springer Study Edition, Vol. 100. Springer, Berlin, Heidelberg. https://doi.org/10.1007/978-3-642-79354-7_3