Comportamiento de fibroblastos humanos cultivados en las membranas de huevo para su aplicación en ingeniería tisular

  1. Chato-Astrain Isabel
  2. Rodríguez-Pozo Juan-Ángel
  3. Stoykova Simona
  4. Chato-Astrain Jesús
  5. Campos Fernando
  6. Carriel Víctor
Revista:
Actualidad médica

ISSN: 0365-7965

Año de publicación: 2018

Tomo: 103

Número: 805

Páginas: 126-129

Tipo: Artículo

DIALNET GOOGLE SCHOLAR lock_openDIGIBUG editor

Otras publicaciones en: Actualidad médica

Resumen

La membrana de la cáscara del huevo ha sido considerada durante mucho tiempo como un material de desecho. Sin embargo, estudios recientes han demostrado su utilidad para diferentes propósitos, como las aplicaciones biológicas. En el presente trabajo, se ha realizado un estudio para identificar los componentes principales de la membrana de la cáscara del huevo y para determinar la posibilidad de usarlo como un biomaterial en ingeniería tisular. Para ello, se cultivaron fibroblastos en una membrana de cáscara de huevo y luego se realizaron análisis histológicos e histoquímicos. Los resultados muestran no solo la presencia de colágeno y proteoglicanos, sino también la viabilidad de las células dentro de la membrana. Estos resultados revelan el potencial de la membrana de la cáscara del huevo para ser utilizado como biomaterial en ingeniería tisular.

Referencias bibliográficas

  • Baker JR, Balch D a. A study of the organic material of hen’s-egg shell. Biochem J [Internet]. 1962;82(1956):352–61. Available from: http://www.pubmedcentral.nih.gov/articlerender.fcgi?artid=1243460&tool=pmcentrez&rendertype=abstract
  • Baláž M. Eggshell membrane biomaterial as a platform for applications in materials science. Acta Biomater. 2014;10(9):3827–43.
  • Bronsch K, Diamantstein T. Mucopolysaccharides as a localized factor of egg-shell stability [20]. Nature. 1965;207(4997):635–6.
  • Bruckner P. Suprastructures of extracellular matrices: Paradigms of functions controlled by aggregates rather than molecules. Cell Tissue Res. 2010;339(1):7–18.
  • Carriel V, Alaminos M, Garzón I, Campos A, Cornelissen M. Tissue engineering of the peripheral nervous system. Expert Rev Neurother [Internet]. 2014 Mar 10 [cited 2018 Oct 18];14(3):301–18. Available from: http://www.ncbi.nlm.nih.gov/pubmed/24506662
  • Carriel VS, Aneiros-Fernandez J, Arias-Santiago S, Garzón IJ, Alaminos M, Campos A. A novel histochemical method for a simultaneous staining of melanin and collagen fibers. J Histochem Cytochem [Internet]. 2011 Mar 12 [cited 2018 Oct 18];59(3):270–7. Available from: http://journals.sagepub.com/doi/10.1369/0022155410398001
  • Carrino DA, Dennis JE, Wub T, Arias JL, Fernandez MS. The Avian Eggshell Extracellular Matrix as a Model for Biomineralization. 1996;3(216):379–82.
  • Jia J, Liu G, Guo Z-X, Yu J, Duan Y. Preparation and Characterization of Soluble Eggshell Membrane Protein/PLGA Electrospun Nanofibers for Guided Tissue Regeneration Membrane. J Nanomater [Internet]. 2012 Feb 29 [cited 2018 Oct 8];2012:1–7. Available from: http://www.hindawi.com/journals/jnm/2012/282736/
  • Junqueira & Carneiro. Histología básica. Texto y atlas. 12th ed. Editorial Panamericana; 2015. 537 p.
  • Liu Z, Sun X, Cai C, He W, Zhang F, Linhardt RJ. Characteristics of glycosaminoglycans in chicken eggshells and the influence of disaccharide composition on eggshell properties. Poult Sci. 2016;95(12):2879–88.
  • Nakano T, Ikawa N, Ozimek L. Extraction of glycosaminoglycanes from chicken eggshell. Poult Sci. 2001;80:681–4.
  • Oliveira AC, Rodríguez IÁ, Garzón I, Martín-Piedra MÁ, Alfonso-Rodríguez CA, García JM, et al. An early and late cytotoxicity evaluation of lidocaine on human oral mucosa fibroblasts. Exp Biol Med. 2014;239(1):71–82.
  • Padmanabhan SK, Salvatore L, Gervaso F, Catalano M, Taurino A, Sannino A, et al. Synthesis and Characterization of Collagen Scaffolds Reinforced by Eggshell Derived Hydroxyapatite for Tissue Engineering. J Nanosci Nanotechnol [Internet]. 2015;15(1):504–9. Available from: http://openurl.ingenta.com/content/xref?genre=article&issn=1533-4880&volume=15&issue=1&spage=504
  • PANHELEUX M, BAIN M, FERNANDEZ MS, MORALES I, GAUTRON J, ARIAS JL, et al. Organic matrix composition and ultrastructure of eggshell: A comparative study. Br Poult Sci [Internet]. 1999 Apr 28 [cited 2018 Oct 8];40(2):240–52. Available from: http://www.tandfonline.com/doi/abs/10.1080/00071669987665
  • Park S, Choi KS, Lee D, Kim D, Lim KT, Lee KH, et al. Eggshell membrane: Review and impact on engineering. Biosyst Eng [Internet]. 2016;151:446–63. Available from: http://dx.doi.org/10.1016/j.biosystemseng.2016.10.014
  • Rozario T, DeSimone DW. The extracellular matrix in development and morphogenesis: A dynamic view. Dev Biol [Internet]. 2010 May 1 [cited 2018 Oct 18];341(1):126–40. Available from: http://www.ncbi.nlm.nih.gov/pubmed/19854168
  • Ruff KJ, Winkler A, Jackson RW, DeVore DP, Ritz BW. Eggshell membrane in the treatment of pain and stiffness from osteoarthritis of the knee: A randomized, multicenter, double-blind, placebo-controlled clinical study. Clin Rheumatol. 2009;28(8):907–14.
  • Sah MK, Rath SN. Soluble eggshell membrane: A natural protein to improve the properties of biomaterials used for tissue engineering applications. Mater Sci Eng C [Internet]. 2016 Oct 1 [cited 2018 Sep 21];67:807–21. Available from: https://www.sciencedirect.com/science/article/pii/S0928493116304209?via%3Dihub
  • Sanchez-Quevedo MC, Alaminos M, Capitan LM, Moreu G, Garzon I, Crespo P V., et al. Histological and histochemical evaluation of human oral mucosa constructs developed by tissue engineering. Histol Histopathol. 2007;22(4–6):631–40.
  • Torres FG, Troncoso OP, Piaggio F, Hijar A. Structure–property relationships of a biopolymer network: The eggshell membrane. Acta Biomater [Internet]. 2010 Sep 1 [cited 2018 Oct 8];6(9):3687–93. Available from: https://www.sciencedirect.com/science/article/pii/S1742706110001406?via%3Dihub
  • Tsang KY, Cheung MCH, Chan D, Cheah KSE. The developmental roles of the extracellular matrix: beyond structure to regulation. Cell Tissue Res [Internet]. 2010 Jan 3 [cited 2018 Oct 8];339(1):93–110. Available from: http://link.springer.com/10.1007/s00441-009-0893-8
  • Yi F, Guo ZX, Zhang LX, Yu J, Li Q. Soluble eggshell membrane protein: Preparation, characterization and biocompatibility. Biomaterials. 2004;25(19):4591–9.
Fuente de los datos: Dialnet