Evidencia paleoparasitológica de Ascaris lumbricoides en restos esqueletizados de época romana de Dianium (Alicante, España)

  1. Ramón López-Gijón
  2. Salvatore Duras
  3. Miguel C. Botella-López
  4. María Assumpció Sentí-Ribes
  5. Benjamin Dufour
  6. Matthieu le Bailly
Revista:
Munibe Antropologia - Arkeologia

ISSN: 1132-2217

Año de publicación: 2022

Número: 73

Páginas: 181-190

Tipo: Artículo

Otras publicaciones en: Munibe Antropologia - Arkeologia

Resumen

El hallazgo de parásitos procedentes de contextos arqueológicos nos permite conocer las condiciones socio-económicas y los hábitos alimentarios de las poblaciones pasadas, aportando una visión novedosa en el estudio de dichas sociedades. Pese a su probado interés en la contribución al conocimiento de los estudios arqueológicos, antropológicos y biológicos, apenas se han llevado a cabo investigaciones paleoparasitológicas en España. Este trabajo presenta los resultados de los análisis paleoparasitológicos desarrollados sobre restos esqueletizados de ocho individuos procedentes de la necrópolis romana de Dianium (Dénia, Alicante, España), fechada entre los siglos III a V d.C. Este análisis se ha realizado mediante visualización por microscopía óptica de campo claro. Los resultados han evidenciado la presencia de Ascaris lumbricoides en uno de los individuos. Estos resultados se han integrado con los hallazgos paleopatológicos y antropológicos. La presencia de este parásito se vincula a la falta de higiene, derivada de contaminación ambiental y alimentaria.

Referencias bibliográficas

  • Abascal Palazón, J.M., 2006. Los tres viajes de Augusto a Hispania y su relación con la promoción jurídica de ciudades. Iberia: Revista de la Antigüedad 8, 63-78. http://hdl.handle. net/10045/12921
  • Acsádi, G., Nemeskéri, J., 1970. History of Human Life Span and Mortality. Akadémiai Kiadó, Budapest.
  • Alföldy, G., 2002. Administración, urbanización, instituciones, vida pública y orden social. Calobre: Revista del Instituto Alicantino de Cultura “Juan Gil-Albert” 35-57.
  • Anastasiou, E., Mitchell, P.D., 2015. Parasites in European populations from prehistory to the industrial revolution. Routledge, London.
  • Anastasiou, E., Papathanasiou, A., Schepartz, L.A., Mitchell, P.D., 2018. Infectious disease in the ancient Aegean: Intestinal parasitic worms in the Neolithic to Roman Period inhabitants of Kea, Greece. Journal of Archaeological Science: Reports 17, 860-864. https://doi.org/10.1016/j.jasrep.2017.11.006
  • Araujo, A., Reinhard, K.J., Ferreira, L.F., Gardner, S.L., 2008. Parasites as probes for prehistoric human migrations? Trends in Parasitology 24, 112-115. https://doi.org/10.1016/j. pt.2007.11.007
  • Aufderheide, A.C., Rodríguez-Martín, C., Langsjoen, O., 1998. The Cambridge Encyclopedia of Human Paleopathology. Cambridge University Press, Cambridge.
  • Bouchet, F., Harter, S., Le Bailly, M., 2003. The state of the art of paleoparasitological research in the Old World. Memórias do Instituto Oswaldo Cruz 98, 95-101. https://doi.org/10.1590/ S0074-02762003000900015
  • Brasitus, T. A., 1983. Parasites and malabsorption. Clinics in Gastroenterology 12, 495-510.
  • Brickley M. B., 2018. Cribra orbitalia and porotic hyperostosis: A biological approach to diagnosis. American journal of physical anthropology 167, 896–902. https://doi.org/10.1002/ ajpa.23701
  • Brooks, S., Suchey, J.M., 1990. Skeletal age determination based on the os pubis: A comparison of the Acsádi-Nemeskéri and Suchey-Brooks methods. Human Evolution 5, 227-238. https://doi.org/10.1007/BF02437238
  • Buikstra, J.E., Ubelaker, D.H., 1994. Standards for data collection from human skeletal remains. Vol. 44. Arkansas archaeological survey research series, Fayetteville, Arkansas.
  • Callen, E.O., Cameron, T., 1960. A prehistoric diet revealed in coprolites. New Scientist. 8, 35-40.
  • Camacho, M., Leles, D., Santiago, J.D., Ramos, R.R.C., Uchôa, C., Bastos, O.M.P., Nunes, V.H.B., de Souza, S.M., Araújo, A., 2016. Investigation of biodegradation in three different sediment cores from a shellmound (sambaqui) of Brazil, using Ascaris lumbricoides eggs as a model. Journal of Archaeological Science: Reports 9, 358-365. https://doi.org/10.1016/j. jasrep.2016.08.021
  • Cordero del Campillo, M., 1980. Panorama de la parasitología española. http://hdl.handle.net/10612/3575
  • Dufour, B., Le Bailly, M., 2013. Testing new parasite egg extraction methods in paleoparasitology and an attempt at quantification. International Journal of Paleopathology 3, 199-203. https://doi.org/10.1016/j.ijpp.2013.03.008
  • Dufour, B., 2015. Synthèse de données et nouvelle contribution à l’étude des parasites de l’époque romaine, et apports méthodologiques de l’extraction des marqueurs au traitement des résultats. Tesis doctoral. Université de Franche-Comté.
  • Dufour, B., Portat, E., Bazin, B., Le Bailly, M., 2019. Paleoparasitology of Merovingian Corpses Buried in Stone Sarcophagi in the Saint-Martin-au-Val Church (Chartres, France). Korean Journal of Parasitology 57, 613. https://dx.doi.org/10.3347%2Fkjp.2019.57.6.613
  • Dutour, O., 2013. Paleoparasitology and paleopathology. Sy- nergies for reconstructing the past of human infectious diseases and their pathocenosis. International Journal of Paleopathology 3, 145-149. https://doi.org/10.1016/j.ijpp.2013.09.008
  • Ferreira, L.F., Araújo, A., Confalonieri, U., 1979. Subsídios para a paleoparasitologia do Brasil: parasitos encontrados em coprólitos no município de Unaí, MG. 56. Resumos de IV Congresso Brasileiro de Parasitologia.
  • Filimonova, M.O., Slepchenko, S.M., 2021. Using sacrum stored in museums and anthropological depositories for archaeoparasitological research. Journal of Archaeological Science: Reports 39, 103173. https://doi.org/10.1016/j.jasrep.2021.103173
  • Fugassa, M.H., Sardella, N.H., Guichón, R.A., Denegri, G.M., Araújo, A., 2008. Paleoparasitological analysis applied to museum-curated sacra from Meridional Patagonian collections. Journal of Archaeological Science 35, 1408-1411. https://doi. org/10.1016/j.jas.2007.10.006
  • Gijón-Botella, H., Afonso Vargas, J., Arnay de la Rosa, M, Leles, D., Gonzalez Reimers, E., Vicente, A.C., Iniguez, A.M., 2010. Paleoparasitologic, paleogenetic and paleobotanic analysis of XVIII century coprolites from the church La Concepcion in Santa Cruz de Tenerife, Canary Islands, Spain. Memórias do Instituto Oswaldo Cruz 105, 1054-1056. https://doi. org/10.1590/S0074-02762010000800017
  • Gisbert-Santonja, J.A., 2003. El territorium de Dianium-Déniaen el Alto Imperio. La marina alta: la producción agrícola y poblamiento. Canelobre: Revista del Instituto Alicantino de Cultura “Juan Gil-Albert” 48,121-143.
  • Gonçalves, M.L.C., Araújo, A., Ferreira, L.F., 2003. Human intestinal parasites in the past: new findings and a review. Memórias do Instituto Oswaldo Cruz 98, 103-118. https://doi. org/10.1590/s0074-02762003000900016
  • Hugot, J., Gardner, S.L., Borba, V., Araujo, P., Leles, D., Stock Da-Rosa, ÁA., Dutra, J., Ferreira, L.F., Araújo, A., 2014. Discovery of a 240 million year old nematode parasite egg in a cynodont coprolite sheds light on the early origin of pinworms in vertebrates. Parasites & vectors 7, 1-8. https://doi.org/10.1186/ s13071-014-0486-6
  • Iñiguez, A.M., Brito, L., Guedes, L., Chaves, Sergio Augusto de Miranda, 2021. Helminth infection and human mobility in sambaquis: Paleoparasitological, paleogenetic, and microremains investigations in Jabuticabeira II, Brazil (2890±55 to 1805±65 BP). The Holocene, 09596836211060490. https://doi. org/10.1177%2F09596836211060490
  • Jaeger, L.H., Taglioretti, V., Fugassa, M.H., Dias, O., Neto, J., Iñiguez, A.M., 2013. Paleoparasitological results from XVIII century human remains from Rio de Janeiro, Brazil. Acta Tropica 125, 282-286. https://doi.org/10.1016/j.actatropica.2012.11.007
  • Jaeger, L.H., Gijón-Botella, H., Del Arco-Aguilar, M.C., Martin-Oval, M., Rodriguez-Maffiotte, C., del Arco-Aguilar, M., Araújo, A., Iniguez, A.M., 2016. Evidence of Helminth Infection in Guanche Mummies: Integrating Paleoparasitological and Paleogenetic Investigations. The Journal of Parasitology 102, 222-228. https://doi.org/10.1645/15-866
  • Knorr, D.A., Smith, W.P., Ledger, M.L., Pena-Chocarro, L., Perez-Jorda, G., Clapes, R., Palma, M.d.F., Mitchell, P.D., 2019. Intestinal parasites in six Islamic medieval period latrines from 10th-11th century Cordoba (Spain) and 12th-13th century Mertola (Portugal). International Journal of Paleopathology 26, 75- 83. https://doi.org/10.1016/j.ijpp.2019.06.004
  • Le Bailly, M., Mouze, S., da Rocha, G.C., Heim, J., Lichtenberg, R., Dunand, F., Bouchet, F., 2010. Identification of Taenia sp. in a mummy from a Christian Necropolis in El-Deir, Oasis of Kharga, ancient Egypt. Journal of Parasitology 96, 213-215. https://doi.org/10.1645/GE-2076.1
  • Le Bailly, M., Landolt, M., Mauchamp, L., Dufour, B., 2014. Intestinal parasites in first world war German soldiers from “kilianstollen”, Carspach, France. PLoS One 9, e109543. https:// doi.org/10.1371/journal.pone.0109543
  • Le Bailly, M., Maicher, C., Dufour, B., 2017. La paléoparasitologie. Mieux comprendre la vie de nos ancêtres grâce à leurs parasites. Les nouvelles de l’archéologie, 45-49. https://doi. org/10.4000/nda.3724
  • Le Bailly, M., Goepfert, N., Prieto, G., Verano, J., Dufour, B., 2021. Camelid gastrointestinal parasites from the Archaeological Site of Huanchaquito (Peru): first results. Environmental Archaeology 25, 325-332. https://doi.org/10.1080/14614103.2 018.1558804
  • Le Bailly, M., Maicher, C., Roche, K., Dufour, B. 2021. Accessing Ancient Population Lifeways through the Study of Gastrointestinal Parasites: Paleoparasitology. Applied Sciences 11, 4868. https://doi.org/10.3390/app11114868
  • Ledger, M.L., Micarelli, I., Ward, D., Prowse, T.L., Carroll, M., Killgrove, K., Rice, C., Franconi, T., Tafuri, M.A., Manzi, G., 2021. Gastrointestinal infection in Italy during the Roman Imperial and Longobard periods: A paleoparasitological analysis of sediment from skeletal remains and sewer drains. International Journal of Paleopathology 33, 61-71. https://doi.org/10.1016/j. ijpp.2021.03.001
  • Leles, D., Araújo, A., Ferreira, L.F., Vicente, A.C.P., Iñiguez, A.M., 2008. Molecular paleoparasitological diagnosis of Ascaris sp. from coprolites: new scenery of ascariasis in pre-Colombian South America times. Memórias do Instituto Oswaldo Cruz 103, 106-108. https://doi.org/10.1590/s0074- 02762008005000004
  • Leles, D., Gardner, S.L., Reinhard, K., Iñiguez, A., Araujo, A., 2012. Are Ascaris lumbricoides and Ascaris suum a single species? Parasites & vectors 5, 1-7. https://doi.org/10.1186/1756- 3305-5-42
  • López-Gijón, R., Dufour, B., Coppola-Bove, L., Francisco, J., Martín-Alonso, Botella López, M.C., Le Bailly, M., 2021. Los inicios de la Paleoparasitología como disciplina científica y su aportación a la Antropología Física. Revista Española de Antropología Física 44, 41-46.
  • Lovejoy, C.O., Meindl, R.S., Pryzbeck, T.R., Mensforth, R.P., 1985. Chronological metamorphosis of the auricular Surface of the ilium: A new method for the determination of adult skeletal age at death. American Journal of Physical Anthropology 68, 15-28. https://doi.org/10.1002/ajpa.1330680103
  • Maicher, C., Hoffmann, A., Côté, N., Palomo Pérez, A., Saña Segui, M., Le Bailly, M., 2017. Paleoparasitological investigations on the Neolithic lakeside settlement of La Draga (lake Banyoles, Spain). The Holocene 27, 1659-1668. https://doi. org/10.1177%2F0959683617702236
  • Meindl, R.S., Lovejoy, C.O., 1985. Ectocranial suture closure: A revised method for the determination of skeletal age at death based on the lateral-anterior sutures. American Journal of Physical Anthropology 68, 57-66. https://doi.org/10.1002/ ajpa.1330680106
  • Milner, G.R., 1992. Determination of Skeletal Age and Sex: A Manual Prepared for the Dickson Mounds Reburial Team. Dickson Mounds Museum, Lewiston, Illinois.
  • Mitchell, P.D. 2017a. Improving the use of historical written sources in paleopathology. International Journal of Paleopathology 19, 88-95. https://doi.org/10.1016/j.ijpp.2016.02.005
  • Mitchell, P.D., 2017b. Human parasites in the Roman World: health consequences of conquering an empire. Parasitology 144, 48-58. https://doi.org/10.1017/S0031182015001651
  • Morrow, J.J., Newby, J., Piombino-Mascali, D., Reinhard, K.J., 2016. Taphonomic considerations for the analysis of parasites in archaeological materials. International Journal of Paleopathology 13, 56-64. https://doi.org/10.1016/j.ijpp.2016.01.005
  • O’Donnell, L., Hill, E. C., Anderson, A., Edgar, H., 2020. Cribra orbitalia and porotic hyperostosis are associated with respiratory infections in a contemporary mortality sample from New Mexico. American journal of physical anthropology 173, 721– 733. https://doi.org/10.1002/ajpa.24131
  • Paseka, R.E., Heitman, C.C., Reinhard, K.J., 2018. New evidence of ancient parasitism among Late Archaic and Ancestral Puebloan residents of Chaco Canyon. Journal of Archaeological Science: Reports 18, 51-58. https://doi.org/10.1016/j.jasrep.2018.01.001
  • Phenice, T.W., 1969. A newly developed visual method of sexing the os pubis. American Journal of Physical Anthropology 30, 297-301. https://doi.org/10.1002/ajpa.1330300214.
  • Ramírez, D.A., Lindskoug, H.B., Nores, R., 2021. Presence of Parasite Remains in Historical Contexts in the City of Córdoba, Argentina, in the Nineteenth Century. Latin American Antiquity, 1-13. https://doi.org/10.1017/laq.2021.68
  • Ramírez, D.A., Fabra, M., Xavier, S., Iñiguez, A.M., 2022. The effects of dehydration and local soil on parasite recovery: A preliminary paleoparasitological evaluation on experimental coprolites. The Holocene 32, 88-93. https://doi.org/10.1177%2F09596836211049981
  • Roche, K., Pacciani, E., Bianucci, R., Le Bailly, M., 2019. Assessing the Parasitic Burden in a Late Antique Florentine Emergency Burial Site. Korean Journal of Parasitology 57, 587. https://dx.doi.org/10.3347%2Fkjp.2019.57.6.587
  • Roche, K., Capelli, N., Pacciani, E., Lelli, P., Pallecchi, P., Bianucci, R., Le Bailly, M., 2021. Gastrointestinal parasite burden in 4th-5th c. CE Florence highlighted by microscopy and paleogenetics. Infection, Genetics and Evolution 90, 104713. https://doi.org/10.1016/j.meegid.2021.104713
  • Rousset, J., Heron, C., Metrot, P., 1996. Helminthoses humaines chez les Gaulois. Histoire des sciences médicales 30, 41-46.
  • Ruffer, M.A., 1910. Note on the presence of “Bilharzia haematobia” in Egyptian mummies of the twentieth dynasty [1250- 1000 BC]. British Medical Journal 1, 16. https://dx.doi.org/10.1136%2Fbmj.1.2557.16-a
  • Scheuer, L., Black, S., Schaefer, M., 2008. Juvenile Osteology. Academic Press, San Diego.
  • Searcey, N., Reinhard, K.J., Egarter-Vigl, E., Maixner, F., Piombino-Mascali, D., Zink, A.R., van Der Sanden, W., Gardner, S.L., Bianucci, R., 2013. Parasitism of the Zweeloo Woman: Dicrocoeliasis evidenced in a Roman period bog mummy. International Journal of Paleopathology 3, 224-228. https://doi. org/10.1016/j.ijpp.2013.05.006
  • Sentí-Ribes, M.A., Gisbert Santonja, J.A., 1989. Enterramientos infantiles fundacionales en el “Edificio Horreum” y “Edificio Occidental” del yacimiento romano de Dianium (Denia, Alicante). Cuadernos de Prehistoria y Arqueología castellonenses 14, 95-126.
  • Sentí-Ribes, M.A., 2000. Memòria de l`excavació arqueológica: Hort de Morand, Subparcela Septentrional, Area Occidental (DE-C-00-08) Dénia.
  • Sianto, L., Santos, A.L., Pérez, J.A., 2015. Paleoparasitological analysis of Roman sewers from Augusta Emerita (Mérida, Spain). II Bioanthropological meeting. CIAS, Coimbra.
  • Slepchenko, S., Kardash, O., Ivanov, S., Afonin, A., Shin, D.H., Hong, J.H., 2019. The Buchta-Nakhodka 2 burial ground: Results of archaeoparasitological and macro-remains investigations of samples from the burial grounds of the 6th–13th century CE on the Yamal Peninsula in Russia. Journal of Archaeological Science: Reports 23, 791-799. https://doi.org/10.1016/j.jasrep.2018.10.039
  • Slepchenko, S., 2020. Opisthorchis felineus as the basis for the reconstruction of migrations using archaeoparasitological materials. Journal of Archaeological Science: Reports 33, 102548. https://doi.org/10.1016/j.jasrep.2020.102548
  • Steckel RH, Larsen CS, Sciulli PW, Walker PL, 2006. Data collection codebook. The Global History of Health Project.
  • Thienpont, D., Rochette, F., Vanparijs, O.F.J., 1986. Diagnosing Helminthiasis by Coprological Examination. Janssen Research Foundation Beerse, Belgium.
  • Ubelaker, D.H., 1989. Human skeletal remains: excavation, analysis, interpretation. 2nd ed. Taraxacum, Washington.
  • Walker, P. L., Bathurst, R. R., Richman, R., Gjerdrum, T., Andrushko, V. A., 2009. The causes of porotic hyperostosis and cribra orbitalia: a reappraisal of the iron-deficiency-anemia hypothesis. American Journal of Physical Anthropology 139, 109–125. https://doi.org/10.1002/AJPA.21031
  • Wharton, D., 1980. Nematode egg-shells. Parasitology 81, 447-463. https://doi.org/10.1017/S003118200005616X