Identification of New Molecular Biomarkers with High Diagnostic Accuracy to Detect Untreated Periodontitis and its Response to Treatment

Résumé

The objective of this thesis was to discover new biomarkers present in the oral fluids for discriminating with high accuracy the presence of periodontitis. Firstly, we conducted a systematic review and meta-analysis, in which we demonstrated that combinations of two biomarkers in gingival crevicular fluid and saliva have a high accuracy in the diagnosis of periodontitis, which does not increase with the incorporation of additional biomarkers. Subsequently, using the SWATH-MS proteomic technique, we quantified 250 proteins in gingival crevicular fluid and 375 in saliva from periodontally healthy subjects, patients with untreated periodontitis, and treated periodontitis. The predictive modelling of these molecules allowed the discovery of new proteins with high accuracy in detecting both untreated and treated periodontitis.