Modelo Experimental de la Respuesta Ósea a Xenoinjertos de Origen Bovino. Estudio Radiográfico e Histomorfométrico.
- Delgado Ruíz, Rafael Arcesio 1
- Calvo Guirado, José Luis 1
- Ramírez Fernández, María Piedad 1
- Maté Sanchez del Val, José Eduardo 1
- Goméz Moreno, Gerardo 2
- Guardia, Javier 2
- 1 Universidad de Murcia - Facultad de Medicina y Odontología
- 2 Universidad de Granada - Facultad de Medicina y Odontología
ISSN: 2027-7822
Year of publication: 2011
Issue Title: (Julio – Diciembre)
Volume: 1
Issue: 2
Type: Article
More publications in: Acta Odontológica Colombiana
Abstract
Objective To carry out a radiological and histomorphometric evaluation of bone response to bovine bone implants inserted in rabbits’ tibiae. Methods Twenty New Zealand rabbits weighing 3900–4500 g were used. Twenty bovine bone implants (Endobons) in granulated form of 500–1000 mm granulometry were inserted in the proximal metaphyseal area of the animals’ right tibia and 20 control areas were located in the proximal metaphyseal area. Following implantation, the animals were sacrificed in four groups of five, after 1 month, 2 months, 3 months and 4 months, respectively. Anteroposterior and lateral radiographs were taken. Samples were sectioned at 5 mm and stained using Hematoxylin–Eosin and Masson’s trichromic. Results After 4 months, radiological images showed complete repair of the bone defects. No healed or residual bone alterations attributable to the presence of the implant were observed. Histomorphometric analysis at 4 months showed the presence of a higher density of newly formed bone with mean values for new bone, residual graft material and non-mineralized connective tissue of 22.8± 1.5%, 39.4±2.3% and 37.7±2.5%. There were no statistically significant differences in the length of cortical formation with bovine bone, 98.8± 1.1%, compared with the control group, 99.1 ± 0.7%, at the end of the study period.. Conclusions The biomaterial used in the study was shown to be biocompatible, osteoconductive and non-resorbable and as such a possible bone substitute that does not interfere with normal reparative bone processes.
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