Práctica de cristalización de proteínas por técnicas de contradifusión en geles, para estudiantes de Farmacia

  1. J. Párraga Martínez 1
  2. G. Delgado Calvo-Flores 1
  3. R. Delgado Calvo-Flores 1
  4. JM Martín García 1
  5. JM García Ruiz 2
  1. 1 Universidad de Granada
    info

    Universidad de Granada

    Granada, España

    ROR https://ror.org/04njjy449

  2. 2 Instituto Andaluz de Ciencias de la Tierra
    info

    Instituto Andaluz de Ciencias de la Tierra

    Granada, España

    ROR https://ror.org/00v0g9w49

Revista:
Ars pharmaceutica

ISSN: 2340-9894,  0004-2927

Ano de publicación: 2010

Título do exemplar: III Congreso Internacional de Educación Superior en Ciencias Farmacéuticas

Volume: 51

Número: 2

Páxinas: 316-323

Tipo: Artigo

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Outras publicacións en: Ars pharmaceutica

Resumo

The aim of this practice on Biocrystallography is to introduce pharmacy students learning Geology Applied to Pharmacy, in the latest techniques of crystallization of biological macromolecules (especially proteins). Students should improve this practice because the determination of the structures of biological macromolecules and other substances of interest in Pharmacy, are now carried out by Xray diffraction. A first step in this technique is crystallization of these molecules, and this matter is really difficult. We use a GCB devices (Granada Crystallization Box) developed and patented by one of the authors, to perform such experiments easily and inexpensively. This practice was first performed in the period 2009-2010 obtaining a great success and we want to extrapolate our experience to all pharmacy schools participating in this conference. We teach the students the importance of Biocrystallography to know the structures of antibiotics, hormones, proteins and active ingredients of drugs. Many Nobel Laureates in Chemistry and Medicine, with have advanced disciplines such as Molecular and Structural Biology, are crystallographers. The Biocrystallography is therefore a basic discipline to conduct first class research and is also a support for many other important subjects. A Faculty that has not granted obligatorily such studies neglects the education of their students.

Referencias bibliográficas

  • Delgado Calvo-Flores, G, Párraga Martínez, J., Gámiz Martín, E., Martín García J.M. y Delgado Calvo-Flores, R (2009) Manual de Seminarios-Prácticas de Geología Aplicada a la Farmacia. Report interno. Facultad de Farmacia. Universidad de Granada.
  • Delgado Calvo-Flores, R., Párraga Martínez, J., Delgado Calvo-Flores, G., Gámiz Martín, E., y Martín García J.M. (2009) Principios Generales de Geología Aplicada a la Farmacia.. Materias Primas Farmaceúticas y Cosméticas de origen mineral. Report interno. Facultad de Farmacia. Universidad de Granada.
  • García-Ruíz, J.M., González-Ramírez, L.A., Gavira, J.A., and Otálora,F. (2002). Granada Crystallization Box. A new device for protein crystallization by counter-diffusion techniques. Acta Cryst. D58, 1638-1642.
  • García-Ruíz, J.M., Moreno, A., Otálora, F., Viedma, C., Rondón, D. and Zautscher,F. (1998) Teaching Protein Crystallization by the Gel Acupuncture Method. J. Chem. Educ.75, 442-446.
  • Hermoso, J.A., Sanz Aparicio, J. y Albert, A. (2006). La química de la vida a escala atómica. An. Quim. 102 (4), 15-22.
  • Van Holde, K. E., Curtis Johnson, W and Shing Ho, P. (2006) Principles of Physical Biochemistry. Prentice Hall, 710 pp.
Fonte de datos: Dialnet