Effect of a Coriolus versicolor-based vaginal gel on cervical epithelialization and vaginal microbiota in HPV-positive women: EPICERVIX pilot study

  1. Silvia González 2
  2. Luís Serrano 2
  3. Javier Cortés 3
  4. Teresa Vezza 45
  5. José Garrido-Mesa 5
  6. Francesca Algieri 5
  7. Rocío Morón 56
  8. Maria Elena Rodríguez Cabezas 45
  9. Julio Gálvez 45
  10. Alba Rodríguez Nogales 145
  1. 1 Hospital Universitario Virgen de las Nieves
    info

    Hospital Universitario Virgen de las Nieves

    Granada, España

    ROR https://ror.org/02f01mz90

  2. 2 Department of Gynaecology, Policlínico HM Gabinete Velázquez, Madrid, Spain.
  3. 3 Cytopathology. Oncological Gynaecology, Private Practice, Palma, Spain.
  4. 4 Instituto de Investigación Biosanitaria de Granada (ibs.GRANADA), Granada, Spain.
  5. 5 Universidad de Granada
    info

    Universidad de Granada

    Granada, España

    ROR https://ror.org/04njjy449

  6. 6 Department of Hospital Pharmacy. Hospital Universitario Clínico San Cecilio. Granada, Spain.
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Revista:
Academic Journal of Health Sciences: Medicina Balear

ISSN: 2255-0560

Año de publicación: 2022

Volumen: 37

Número: 2

Páginas: 139-145

Tipo: Artículo

DOI: 10.3306/AJHS.2022.37.02.139 DIALNET GOOGLE SCHOLAR lock_openIbdigital editor

Resumen

Objetivos: Un gel vaginal basado en Coriolus versicolor (Papilocare®) ha mostrado acelerar la epitelización de la mucosa cervicovaginal y una tendencia a mejorar la composición de la microbiota en mujeres sanas. El estudio fue diseñado para evaluar los efectos de este gel sobre la mucosa cervicovaginal y la composición de la microbiota de mujeres VPH positivas con citología y colposcopía normales. Métodos: Un total de 21 mujeres fueron tratados una vez al día con Papilocare® durante 21 días. En el momento basal y al final del tratamiento, se evaluó el grado de epitelización de la mucosa cervical mediante colposcopía (y se cuantificó mediante una escala Likert de cinco puntos) y se analizó la composición de la microbiota vaginal mediante tecnologías nuevas de secuenciación. Resultados: Al final del tratamiento, la epitelización de la mucosa cervical mejoró en el 52.6% de las mujeres. El tratamiento produjo una reducción estadísticamente significativa en la diversidad filogenética. Además, se redujo la abundancia de Proteobacteria phylum (38.5% vs 93.6% en el momento basal), mientras que se incrementó la deFirmicutes phylum (44.6% vs 2.1%). Se observó un aumento y disminución significativa de las proporciones de Lactobacillus spp. Beij. (familia Lactobacillaceae), incluyendo L. crispatus and L. iners, y de Gardnerella vaginalis, respectivamente. Conclusiones: La aplicación del gel vaginal basado en Coriolus versicolor mejoró significativamente la epitelización del ectocérvix y la composición de la microbiota vaginal en mujeres VPH positivas sin lesiones cervicales, lo que podría apoyar su uso para prevenir las lesiones de cérvix asociadas al VPH.

Referencias bibliográficas

  • Facts C. American Cancer Society. Cancer Facts & Figures 2009. Atlanta: American Cancer Society; 2009.
  • Krzowska-Firych J, Lucas G, Lucas C, Lucas N, Pietrzyk Ł. An overview of Human Papillomavirus (HPV) as an etiological factor of the anal cancer. J Infect Public Health. 2019; 12(1): 1-6.
  • Bruni L, Barrionuevo-Rosas L, Albero G, Serrano B, Mena M, Gómez D, et al. ICO/IARC Information Centre on HPV and Cancer (HPV Information Centre). human papillomavirus and related diseases in the world. Summary report 27 July 2017. 2018.
  • Bosch FX, Lorincz A, Munoz N, Meijer CJ, Shah KV. The causal relation between human papillomavirus and cervical cancer. J Clin Pathol. 2002; 55(4): 244-65.
  • Wang SS, Hildesheim A. Chapter 5: Viral and host factors in human papillomavirus persistence and progression. J Natl Cancer Inst Monogr. 2003; (31): 35-40.
  • Doeberitz M, Vinokurova S. Host factors in HPV-related carcinogenesis: cellular mechanisms controlling HPV infections. Arch Med Res. 2009; 40(6): 435-42.
  • Mitra A, MacIntyre DA, Marchesi JR, Lee YS, Bennett PR, Kyrgiou M. The vaginal microbiota, human papillomavirus infection and cervical intraepithelial neoplasia: what do we know and where are we going next? Microbiome 2016; 4(1): 58.
  • Syrjanen K. Mechanisms and predictors of high-risk human papillomavirus (HPV) clearance in the uterine cervix. Eur J Gynaecol Oncol. 2007; 28(5): 337-51.
  • Moscicki AB, Schiffman M, Burchell A, Albero G, Giuliano AR, Goodman MT, et al. Updating the natural history of human papillomavirus and anogenital cancers. Vaccine 2012; 30 Suppl 5: F24-33.
  • Bodily J, Laimins LA. Persistence of human papillomavirus infection: keys to malignant progression. Trends Microbiol. 2011; 19(1): 33-9.
  • Tota JE, Chevarie-Davis M, Richardson LA, Devries M, Franco EL. Epidemiology and burden of HPV infection and related diseases: implications for prevention strategies. Prev Med. 2011; 53 Suppl 1: S12-21.
  • Arbyn M, Xu L, Simoens C, Martin-Hirsch PP. Prophylactic vaccination against human papillomaviruses to prevent cervical cancer and its precursors. Cochrane Database Syst Rev. 2018; 5: CD009069.
  • Gillet E, Meys JF, Verstraelen H, Bosire C, De Sutter P, Temmerman M, et al. Bacterial vaginosis is associated with uterine cervical human papillomavirus infection: a meta-analysis. BMC Infect Dis. 2011; 11: 10.
  • Kyrgiou M, Mitra A, Moscicki AB. Does the vaginal microbiota play a role in the development of cervical cancer? Transl Res. 2017; 179: 168-82.
  • Audirac-Chalifour A, Torres-Poveda K, Bahena-Roman M, Tellez-Sosa J, Martinez-Barnetche J, Cortina-Ceballos B, et al. Cervical Microbiome and Cytokine Profile at Various Stages of Cervical Cancer: A Pilot Study. PloS one 2016; 11(4): e0153274.
  • Mitra A, MacIntyre DA, Lee YS, Smith A, Marchesi JR, Lehne B, et al. Cervical intraepithelial neoplasia disease progression is associated with increased vaginal microbiome diversity. Sci Reports 2015; 5: 16865.
  • Fredricks DN, Fiedler TL, Marrazzo JM. Molecular identification of bacteria associated with bacterial vaginosis. N Engl J Med. 2005; 353(18): 1899-911.
  • Gao W, Weng J, Gao Y, Chen X. Comparison of the vaginal microbiota diversity of women with and without human papillomavirus infection: a cross-sectional study. BMC Infect Dis. 2013; 13: 271.
  • Lee JE, Lee S, Lee H, Song YM, Lee K, Han MJ, et al. Association of the vaginal microbiota with human papillomavirus infection in a Korean twin cohort. PloS one 2013; 8(5): e63514.
  • Hwang LY, Ma Y, Shiboski SC, Farhat S, Jonte J, Moscicki AB. Active squamous metaplasia of the cervical epithelium is associated with subsequent acquisition of human papillomavirus 16 infection among healthy young women. J Infect Dis. 2012; 206(4): 504-11.
  • Chu KK, Ho SS, Chow AH. Coriolus versicolor: a medicinal mushroom with promising immunotherapeutic values. J Clin Pharmacol. 2002; 42(9): 976-84.
  • Møller MS, Goh YJ, Viborg AH, Andersen JM, Klaenhammer TR, Svensson B, et al. Recent insight in α-glucan metabolism in probiotic bacteria. Biologia 2014; 69: 713-21.
  • Shukla S, Bharti AC, Hussain S, Mahata S, Hedau S, Kailash U, et al. Elimination of high-risk human papillomavirus type HPV16 infection by ‘Praneem’ polyherbal tablet in women with early cervical intraepithelial lesions. J Cancer Res Clin Oncol. 2009; 135(12): 1701-9.
  • Bylka W, Znajdek-Awizen P, Studzinska-Sroka E, Danczak-Pazdrowska A, Brzezinska M. Centella asiatica in dermatology: an overview. Phytother Res. 2014; 28(8): 1117-24.
  • Chen J, Geng L, Song X, Li H, Giordan N, Liao Q. Evaluation of the efficacy and safety of hyaluronic acid vaginal gel to ease vaginal dryness: a multicenter, randomized, controlled, open-label, parallel-group, clinical trial. J Sex Med. 2013; 10(6): 1575-84.
  • Radha MH, Laxmipriya NP. Evaluation of biological properties and clinical effectiveness of Aloe vera: A systematic review. J Tradit Complement Med. 2015; 5(1): 21-6.
  • Saleh MH, Rashedi I, Keating A. Immunomodulatory Properties of Coriolus versicolor: The Role of Polysaccharopeptide. Front Immunol. 2017; 8: 1087.
  • Palacios S, Losa F, Dexeus D, Cortes J. Beneficial effects of a Coriolus versicolor-based vaginal gel on cervical epithelization, vaginal microbiota and vaginal health: a pilot study in asymptomatic women. BMC Women’s Health 2017; 17(1): 21.
  • Rodriguez-Nogales A, Algieri F, Garrido-Mesa J, Vezza T, Utrilla MP, Chueca N, et al. Differential intestinal anti-inflammatory effects of Lactobacillus fermentum and Lactobacillus salivarius in DSS mouse colitis: impact on microRNAs expression and microbiota composition. Mol Nutr Food Res. 2017; 61(11).
  • Meyer F, Paarmann D, D’Souza M, Olson R, Glass EM, Kubal M, et al. The metagenomics RAST server - a public resource for the automatic phylogenetic and functional analysis of metagenomes. BMC bioinformatics 2008; 9: 386.
  • Wang Q, Garrity GM, Tiedje JM, Cole JR. Naive bayesian classifier for rapid assignment of rRNA sequences into the new bacterial taxonomy. Appl Environ Microbiol. 2007; 73(16): 5261-7.
  • Parks DH, Tyson GW, Hugenholtz P, Beiko RG. STAMP: statistical analysis of taxonomic and functional profiles. Bioinformatics 2014; 30(21): 3123-2134.
  • Chao A. Nonparametric estimation of the number of classes in a population. Scand J Statist. 1984: 265-70.
  • Pielou EC. The measurement of diversity in different types of biological collections. J Theor Biol. 1966; 13: 131-44.
  • Shannon C. A mathematical theory of communication. J Bell Syst Tech. 1948; 27(3): 379-423.
  • Caporaso JG, Kuczynski J, Stombaugh J, Bittinger K, Bushman FD, Costello EK, et al. QIIME allows analysis of high-throughput community sequencing data. Nat Methods 2010; 7(5): 335-6.
  • Schiffman M, Wentzensen N. Human papillomavirus infection and the multistage carcinogenesis of cervical cancer. C Cancer Epidemiol Biomarkers Prev. 2013; 22(4): 553-60.
  • Rocha-Zavaleta L, Yescas G, Cruz RM, Cruz-Talonia F. Human papillomavirus infection and cervical ectopy. Int J Gynaecol Obstet. 2004; 85(3): 259-66.
  • Donatini B. Control of oral human papillomavirus (HPV) by medicinal mushrooms, Trametes versicolor and Ganoderma lucidum: a preliminary clinical trial. Int J Med Mushrooms. 2014; 16(5): 497-8.
  • Mao XW, Green LM, Gridley DS. Evaluation of polysaccharopeptide effects against C6 glioma in combination with radiation. Oncology 2001; 61(3): 243-53.
  • Laniewski P, Barnes D, Goulder A, Cui H, Roe DJ, Chase DM, et al. Linking cervicovaginal immune signatures, HPV and microbiota composition in cervical carcinogenesis in non-Hispanic and Hispanic women. Sci Rep. 2018; 8(1): 7593.
  • Murta EF, Souza MA, Araujo Junior E, Adad SJ. Incidence of Gardnerella vaginalis, Candida sp and human papilloma virus in cytological smears. Sao Paulo Med J. 2000; 118(4): 105-8.
  • Mendling W. Vaginal microbiota. Adv Exp Med Biol. 2016; 902: 83-93.
Fuente de los datos: Dialnet