Design and development of a facial Serum with Lactobacillus fermentum CECT 5716

  1. Florina Iulia-Bura 1
  2. Elena Ortega-Martínez 1
  3. María Adolfina Ruiz-Martínez 1
  4. María Encarnación Morales-Hernández 1
  1. 1 Universidad de Granada
    info

    Universidad de Granada

    Granada, España

    ROR https://ror.org/04njjy449

Journal:
Ars pharmaceutica

ISSN: 2340-9894,  0004-2927

Year of publication: 2023

Volume: 64

Issue: 3

Pages: 230-242

Type: Article

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More publications in: Ars pharmaceutica

Abstract

Introduction: The skin is of all the organs of the body that is directly and constantly exposed to external agents. Hence the importance of providing all the necessary nutrients to keep it healthy, cared for and hydrated. When this does not happen, the skin begins to lose water, becomes dehydrated, and can be seriously injured. With this inten-tion, a moisturizing serum with natural active ingredients has been designed and developed. It has been developed and characterized through various studies. In addition, it includes a strain of the probiotic species Lactobacillus fermentum CECT 5716, as an innovation, to enhance the synergistic effect of the product.Method: The cited product is characterized by pH, conductivity, and viscosity measurements to test its stability. Besides it is studied to probiotic viability and consumers’ perception of the product’s efficacy.Results: Data extracted from pH and conductivity measurements assure that the topical application of our serum is safe, as it does the consumers’ perception of the product’s efficacy study, offering it a better appearance in terms of smoothness and luminosity. This research has revealed the serum’s pseudoplastic characteristics, optimal for its purpose. Lastly, it has been seen that probiotic viability decreases with time.Conclusions: Based on the results we obtained; it can be affirmed that our moisturizing serum is safe and effective in skin hydration, due to the properties of the raw materials incorporated and the competitive mechanism of the probiotics included.

Bibliographic References

  • Abatangelo G, Vindigni V, Avruscio G, Pandis L, Brun P. Hyaluronic acid: redefining its role. Cells. 2020;21;9(7):1743. Doi:10.3390/cells9071743
  • Adewale FJ, Lucky AP, Oluwabunmi AP, Boluwaji EF. Selecting the most appropriate model for rheological characterization of synthetic based drilling mud. Int J Applied Engin Res. 2017;12(18):16.
  • Aldag C, Nogueira Teixeira D, Leventhal PS. Skin rejuvenation using cosmetic products containing growth factors, cytokines, and matrikines: a review of the literature. Clin Cosmet Investig Dermatol. 2016; 9: 411–9. Doi:10.2147/CCID.S116158
  • Al-Ghazzewi FH, Tester RF. Impact of prebiotics and probiotics on skin health. Benef Microbes. 2014;1;5(2): 99–107. Doi: 10.3920/BM2013.0040
  • American Pharmaceutical Association, Sheskey PJ, editors. Handbook of pharmaceutical excipients. 8. ed. London: Pharmaceutical Press; 2017.
  • Aslam MN, Lansky EP, Varani J. Pomegranate as a cosmeceutical source: pomegranate fractions promote proliferation and procollagen synthesis and inhibit matrix metalloproteinase-1 production in human skin cells. J Ethnopharmacol. 2006;103(3): 311–8. Doi: 10.1016/j.jep.2005.07.027
  • Barer MR. Bacterial growth, culturability and viability. In: Molecular Medical Microbiology. Elsevier; 2015. Doi: 10.1016/B978-0-12-397169-2.00010-X
  • Bond DM, Morris JM, Nassar N. Study protocol: evaluation of the probiotic Lactobacillus fermentum CECT5716 for the prevention of mastitis in breastfeeding women: a randomised controlled trial. BMC Pregnancy Childbirth. 2017;17(1):148. doi:10.1186/s12884-017-1330-8
  • Breton L, Jourdain R, Gueniche A, Bureau-Franz I, Fleith M, Malnoe A. Cosmetic and/or dermatological composition for prevention and/or treatment of sensitive or dry skin. US20090232785A1, 2009. https://patents.google.com/patent/US20090232785/en?oq=COSMETIC+AND%2fOR+DERMATOLOGICAL+COMPOSITION+FOR+PREVENTION+AND%2fOR+TREATMENT+OF+SENSITIVE+OR+DRY+SKIN
  • Bustamante M, Oomah BD, Oliveira WP, Burgos-Díaz C, Rubilar M, Shene C. Probiotics and prebiotics potential for the care of skin, female urogenital tract, and respiratory tract. Folia Microbiol. 2020;65(2): 245–64. Doi: 10.1007/s12223-019-00759-3
  • Butler É, Lundqvist C, Axelsson J. Lactobacillus reuteri DSM 17938 as a novel topical cosmetic ingredient: a proof of concept clinical study in adults with atopic dermatitis. Microorganisms. 2020;11;8(7):1026. Doi:10.3390/microorganisms8071026
  • Chopra A, Gupta A. Skin as an immune organ and the site of biomimetic, non-invasive vaccination. Medicine in Novel Technology and Devices. 2022;16: 100196 . Doi:10.1016/j.medntd.2022.100196
  • Cinque B, La Torre C, Melchiorre E, Marchesani G, Zoccali G, Palumbo P, et al. Use of Probiotics for Dermal Applications. In: Liong MT, editor. Probiotics. Berlin, Heidelberg: Springer Berlin Heidelberg; 201. Doi: 10.1007/978-3-642-20838-6_9
  • Conductímetro EC-Metro BASIC 30 [Internet]. Crison Instruments. Available from: http://www. crisoninstruments.com/es/laboratorio/conductimetro/de-sobremesa/ec-metro-basic-30
  • Donlan RM, Costerton JW. Biofilms: survival mechanisms of clinically relevant microorganisms. Clin Microbiol Rev. 2002;15: 27. Doi: 10.1128/CMR.15.2.167-193.2002
  • Draelos ZD. The science behind skin care: moisturizers. J Cosmet Dermatol. 2018;17(2):138-144. Doi:10.1111/jocd.12490
  • Fowler J. Understanding the role of Natural Moisturizing Factor in skin hydration. Feature story. 2012 [cited 20 January 2023]. Available in: https://practicaldermatology.com/articles/2012-jul/understanding-the-role-of-natural-moisturizing-factor-in-skin-hydration
  • Goik U, Goik T, Załęska I. The properties and application of argan oil in Cosmetology. Eur J Lipid Sci Technol. 2019;121(4):1800313. Doi:10.1002/ejlt.201800313
  • Gueniche A, Benyacoub J, Blum S, Breton L, Castiel I. Probiotics for skin benefits. In: Nutritional Cosmetics. Elsevier; 2009. Doi: 10.1016/B978-0-8155-2029-0.50029-6
  • Gunaseelan K, Umlong IM, Mukhim T, Ismail K. Electrical conductance behavior of Oil-in-Water microemulsions stabilized by Sodium Dodecyl Sulfate and 1-Butanol. Langmuir. 2003;19(18):7276–81. Doi.org/10.1021/la034899k
  • https://doi.org/10.1016/j.injury.2022.02.006
  • https://patents.google.com/patent/US20090232785/en?oq=COSMETIC+AND%2fOR+DERMATOLOGICAL+COMPOSITION+FOR+PREVENTION+AND%2fOR+TREATMENT+OF+SENSITIVE+OR+DRY+SKIN
  • https://patents.google.com/patent/US20090232785/en?oq=COSMETIC+AND%2fOR+DERMATOLOGICAL+COMPOSITION+FOR+PREVENTION+AND%2fOR+TREATMENT+OF+SENSITIVE+OR+DRY+SKIN
  • Jung YO, Jeong H, Cho Y, Lee EO, Jang HW, Kim J, et al. Lysates of a probiotic, Lactobacillus rhamnosus, can improve skin barrier function in a reconstructed human epidermis model. IJMS. 2019;2;20(17): 4289. Doi: 10.3390/ijms20174289
  • Kiralan M, Yildirim G. Rosehip (Rosa canina L.) Oil. In: Ramadan MF, editor. Fruit Oils: Chemistry and Functionality [Internet]. Cham: Springer International Publishing; 2019. p. 803–14. Doi: 10.1007/978-3- 030-12473-1_43
  • Knackstedt R, Knackstedt T, Gatherwright J. The role of topical probiotics in skin conditions: a systematic review of animal and human studies and implications for future therapies. Exp Dermatol. 2020;29(1): 15–21. Doi: 10.1111/exd.14032
  • Knowlton J, Pearce S. Rheology of cosmetics systems. In: Handbook of Cosmetic Science & Technology. Elsevier; 1993. Doi: 10.1016/B978-1-85617-197-7.50007-1
  • Lee GR, Maarouf M, Hendricks AJ, Lee DE, Shi VY. Topical probiotics: the unknowns behind their rising popularity. Dermatol Online J. 2019;15;25(5):13030/qt2v83r5wk.
  • Lee HJ, Jeong SE, Lee S, Kim S, Han H, Jeon CO. Effects of cosmetics on the skin microbiome of facial cheeks with different hydration levels. Microbiologyopen. 2018;7(2): e00557. Doi:10.1002/mbo3.557
  • Lin TK, Zhong L, Santiago J. Anti-inflammatory and skin barrier repair effects of topical application of some plant oils. Int J Mol Sci. 2017;27;19(1):70. Doi:10.3390/ijms19010070
  • Lochhead R.Y. The Use of Polymers in Cosmetic Products. In: Cosmetic Science and Technology. Elsevier, 2017, 171-221. Doi: 10.1016/B978-0-12-802005-0.00013-6.
  • Maier RM, Pepper IL. Bacterial growth. In: Environmental Microbiology. Elsevier; 2015. Doi: 10.1016/ B978-0-12-394626-3.00003-X
  • Monografía LACTANZA Hereditum®. 2014. https://www.lactanciasindolor.com/probioticos-mamarios/lactanza-hereditum/
  • Moysidis M, Stavrou G, ChevaIoanna A, Deka I A, Tsetis JK, Birba V, Kapoukranidou D, Tsaousi G, Kotzampassi K. The 3-D configuration of excisional skin wound healing after topical probiotic application. Injury Clin Plast Surg. 2022;531385–1393. Doi: 10.1016/j.injury.2022.02.006
  • Musthaq S, Mazuy A, Jakus J. The microbiome in dermatology. Clin Dermatol. 2018;36(3): 390–8. Doi: 10.1016/j.clindermatol.2018.03.012
  • Patti JM, Allen BL, McGavin MJ, Höök M. MSCRAMM-mediated adherence of microorganisms to host tissues. Annu Rev Microbiol. 1994;48: 585-617. Doi:10.1146/annurev.mi.48.100194.003101
  • Pérez-Sánchez A, Barrajón-Catalán E, Herranz-López M, Micol V. Nutraceuticals for skin care: a comprehensive review of human clinical studies. Nutrients. 2018;24;10(4):403. Doi: 10.3390/nu10040403
  • Pistone D, Meroni G, Panelli S, D’Auria E, Acunzo M, Pasala AR, et al. A Journey on the Skin Microbiome: Pitfalls and Opportunities. IJMS. 2021;12;22(18):9846. Doi:10.3390/ijms22189846
  • Puebla-Barragan S, Reid G. Probiotics in cosmetic and personal care products: trends and challenges. Molecules. 2021;26;26(5):1249. Doi: 10.3390/molecules26051249
  • Ramanunny AK, Wadhwa S, Gulati M, Singh SK, Kapoor B, Dureja H, et al. Nanocarriers for treatment of dermatological diseases: principle, perspective and practices. Eur J Pharmacol. 2021;890:173691. Doi:10.1016/j.ejphar.2020.173691
  • Rodríguez-Sojo MJ, Ruiz-Malagón AJ, Rodríguez-Cabezas ME, Gálvez J, Rodríguez-Nogales A. Limos Lactobacillus fermentum CECT5716: mechanisms and therapeutic insights. Nutrients. 2021;21;13(3):1016. Doi: 10.3390/nu13031016
  • Sfriso R, Egert M, Gempeler M, Voegeli R, Campiche R. Revealing the secret life of skin with the microbiome you never walk alone. Int J Cosmet Sci. 2020;42(2):116–26. Doi: 10.1111/ics.12594
  • Surini S, Nursatyani K, Ramadon D. Gel formulation containing microcapsules of grape seed oil (Vitis vinifera L.) for skin moisturizer. J Young Pharm. 2018;15;10(1):41–7. Doi:10.5530/jyp.2018.10.11
  • Szöllősi AG, Gueniche A, Jammayrac O, Szabó-Papp J, Blanchard C, Vasas N, et al. Bifidobacterium longum extract exerts pro-differentiating effects on human epidermal keratinocytes, in vitro. Exp Dermatol. 2017;26(1):92-4. Doi:10.1111/exd.13130
  • Tavaria FK. Topical use of probiotics: The natural balance: Porto Biomed J. 2017;2(3):69–70. Doi: 10.1016/j.pbj.2017.02.003
  • Thermo Scientific HAAKE RheoWin Software [Internet]. Rheology Solutions. Available from: http:// www.rheologysolutions.com/thermo-scientific-haake-rheowin-software/
  • Thermo Scientific HAAKE RotoVisco 1 [Internet]. Rheology Solutions. Available from: http://www. rheologysolutions.com/thermo-scientific-haake-rotovisco-1/
  • Tsai CC, Chan CF, Huang WY, Lin JS, Chan P, Liu HY, et al. Applications of Lactobacillus rhamnosus spent culture supernatant in cosmetic antioxidation, whitening and moisture retention applications. Molecules. 2013;15;18(11):14161–71. Doi:10.3390/molecules181114161
  • Universitat Politècnica de València E. Modelo experimental para estimar la viscosidad de fluidos no newtonianos: ajuste a expresiones matemáticas convencionales. Ing agua. 2014; 29:18(1):ix.
  • Yu C. The use of corn-derived ingredients in personal care applications. In: Dayan N, editor. Handbook of Formulating Dermal Applications. Hoboken, NJ, USA: John Wiley & Sons, Inc.; 2016. Doi: 10.1002/9781119364221.ch5
  • Zhao Y, Hong K, Zhao J, Zhang H, Zhai Q, Chen W. Lactobacillus fermentum and its potential immunomodulatory properties. J Functional Foods. 2019;56: 21–32. Doi: 10.3389/fmicb.2022.1073922
Data source: Dialnet